癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC-P, IHC-F |
|---|---|
| Primary Accession | Q5BJY9 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Human, Rat |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for Cytokeratin Peptide 18, keratin 18 (KRT18) detection. Tested with WB, IHC-P, IHC-F in Human; rat. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 294853 |
|---|---|
| Other Names | Keratin, type I cytoskeletal 18, Cytokeratin-18, CK-18, Keratin-18, K18, Krt18 {ECO:0000250|UniProtKB:P05783} |
| Calculated MW | 47761 Da |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 1-2 µg/ml, Human, rat, By Heat Immunohistochemistry(Frozen Section), 0.5-2 µg/ml, Human, rat, - Western blot, 0.5-1 µg/ml, Human, rat |
| Subcellular Localization | Nucleus matrix . Nucleus, nucleolus . Cytoplasm . |
| Tissue Specificity | Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue. . |
| Source | Eukaryota |
| Protein Name | Keratin, type I cytoskeletal 18 |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | CY-90 |
| Immunogen | The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the intermediate filament family. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Krt18 {ECO:0000250|UniProtKB:P05783} |
|---|---|
| Function | Structural component of intermediate filaments in epithelial cells. Forms obligate heteropolymers with a type II keratin KRT8, mechanical stability and resilience to epithelial cells (PubMed:1691189). KRT8/KRT18 filaments are involved in ARHGEF40- mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (By similarity). Organization and orientation of KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules (By similarity). Also acts downstream of ROCK kinase activation as part of a positive feedback mechanism in response to cellular mechanical stress loading (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (PubMed:9353322). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection (By similarity). |
| Cellular Location | Nucleus matrix. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:P05783}. Nucleus, nucleolus {ECO:0000250|UniProtKB:P05783}. Cytoplasm |
| Tissue Location | Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Intermediate filaments(IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18(KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.
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