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Anti-GAP43 Antibody (Monoclonal, GAP-7B10)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC-P, IHC-F |
|---|---|
| Primary Accession | P07936 |
| Host | Mouse |
| Isotype | Mouse IgG2a |
| Reactivity | Human, Mouse, Rat |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for GAP43, growth associated protein 43 (GAP43) detection. Tested with WB, IHC-P, IHC-F in Human; mouse; rat. No cross reactivity with otherproteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 29423 |
|---|---|
| Other Names | Neuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, Protein F1, Gap43 |
| Calculated MW | 23603 Da |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 1-2 µg/ml, Human, mouse, rat, By Heat Immunohistochemistry(Frozen Section), 1-2 µg/ml, Human, mouse, rat, - Western blot, 0.5-1 µg/ml, Human, mouse, rat |
| Subcellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane ; Peripheral membrane protein . Cytoplasmic surface of growth cone and synaptic plasma membranes. |
| Source | Eukaryota |
| Protein Name | Neuromodulin |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | GAP-7B10 |
| Immunogen | GAP-43 from neonatal rat forebrain membranes. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the neuromodulin family. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Gap43 |
|---|---|
| Function | This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction (By similarity). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P17677}; Peripheral membrane protein {ECO:0000250|UniProtKB:P17677}; Cytoplasmic side {ECO:0000250|UniProtKB:P17677}. Cell projection, growth cone. Cell projection, growth cone membrane {ECO:0000250|UniProtKB:P17677}; Peripheral membrane protein {ECO:0000250|UniProtKB:P17677}; Cytoplasmic side {ECO:0000250|UniProtKB:P17677}. Synapse {ECO:0000250|UniProtKB:P17677} Cell projection, filopodium membrane {ECO:0000250|UniProtKB:P17677}; Peripheral membrane protein {ECO:0000250|UniProtKB:P17677}. Perikaryon. Cell projection, dendrite. Cell projection, axon. Cytoplasm Note=Cytoplasmic surface of growth cone and synaptic plasma membranes {ECO:0000250|UniProtKB:P17677} |
| Tissue Location | Expressed in hippocampal neurons, with highest levels of expression in the CA4 and CA3 neurons and lower levels in CA1 neurons (PubMed:11948657, PubMed:17577668). Expressed in the dorsal root ganglion (PubMed:12957493). |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system.
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