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为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-P-GlycoProtein(MDR) Antibody (Monoclonal, F4)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC-P, IHC-F, ICC |
|---|---|
| Primary Accession | P21447 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Human |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for P-Glycoprotein (MDR), ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1 ) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 18671 |
|---|---|
| Other Names | Multidrug resistance protein 1A, 3.6.3.44, ATP-binding cassette sub-family B member 1A, MDR1A, Multidrug resistance protein 3, P-glycoprotein 3, Abcb1a, Abcb4, Mdr1a, Mdr3, Pgy-3, Pgy3 |
| Calculated MW | 140647 Da |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 1-2 µg/ml, Human, By Heat Immunocytochemistry , 1 µg/ml, Human, - Immunohistochemistry(Frozen Section), 1-2 µg/ml, Human, - Western blot, 0.5-1 µg/ml, Human |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
| Tissue Specificity | ABCB1: Expressed in liver, kidney, small intestineand brain. |
| Source | Eukaryota |
| Protein Name | Multidrug resistance protein 1 |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | F4 |
| Immunogen | Mixture of human and hamster drug-resistant whole cells and crude plasma membranes. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Abcb1a {ECO:0000312|MGI:MGI:97570} |
|---|---|
| Function | Translocates drugs and phospholipids across the membrane. Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D- glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug- resistant cells (By similarity). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P08183}; Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441} Apical cell membrane. Cytoplasm {ECO:0000250|UniProtKB:P08183}. Note=ABCB1 localization is influenced by C1orf115 expression levels (plasma membrane versus cytoplasm) {ECO:0000250|UniProtKB:P08183} |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
P-Glycoprotein,, also known as Multidrug Resistance 1(MDR1), is one of the ATP-binding cassette transporters family. P-glycoprotein-1 is involved in the transport of 3 of these protease inhibitors in vitro. MDR1 gene is mapped to the 7q21.1 by in situ hybridization. The MDR1 gene product, P-glycoprotein, mediates the transport of the cardiac glycoside, digoxin.
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