癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-Nebulin Antibody (Monoclonal, NB2)
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- 实验流程
- 背景知识
Application 
| WB |
|---|---|
| Primary Accession | P20929 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for Nebulin, nebulin (NEB) detection. Tested with WB in chicken. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 4703 |
|---|---|
| Other Names | Nebulin, NEB |
| Calculated MW | 986665 Da |
| Application Details | Western blot, 1-2 µg/ml, Chicken |
| Subcellular Localization | Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton. |
| Tissue Specificity | Muscle specific. Located in the thin filament of striated muscle. |
| Source | Eukaryota |
| Protein Name | Nebulin |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | NB2 |
| Immunogen | Chicken breast nebulin. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Contains 178 nebulin repeats. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | NEB |
|---|---|
| Function | This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin. |
| Cellular Location | Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton |
| Tissue Location | Expressed in skeletal muscle (at protein level) (PubMed:23985323). Located in the thin filament of striated muscle (PubMed:9514727). |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Nebulin is a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-, and developmental stage-specific. The nebulin gene contains 183 exons in a 249-kb genomic region. Nebulin is mapped to chromosome 2. Nebulin is a giant filamentous protein specific for vertebrate skeletal muscles. Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.
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