癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-NEFH Antibody (Monoclonal, N52)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC-P, IHC-F |
|---|---|
| Primary Accession | P16884 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Human, Mouse, Rat |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for NEFH, neurofilament, heavy polypeptide (NEFH) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Other Names | Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein, Nefh, Nfh |
|---|---|
| Calculated MW | 115378 Da |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 1-2 µg/ml, Human, mouse, rat, By Heat Immunohistochemistry(Frozen Section), 1-2 µg/ml, Human, mouse, rat, - Western blot, 0.5ml, Human, mouse, rat |
| Source | Eukaryota |
| Protein Name | Neurofilament heavy polypeptide |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | N52 |
| Immunogen | C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the intermediate filament family. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Nefh |
|---|---|
| Synonyms | Nfh |
| Function | Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NEF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity). |
| Cellular Location | Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P19246}. Cell projection, axon {ECO:0000250|UniProtKB:P19246} |
| Tissue Location | Expressed in the dorsal root ganglion neurons (at protein level) (PubMed:9388258). Expressed in cutaneous and muscular sensory neurons (PubMed:19913522). |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD(NFL), 125 kD(NFM), and 200 kD(NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein(NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions(exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease(CMT).
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