癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-Vimentin Antibody (Monoclonal, V9)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | P31000 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Human, Rat, Pig |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for Vimentin, vimentin (VIM) detection. Tested with WB, IHC-P in Human;rat;rabbit;pig. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 81818 |
|---|---|
| Other Names | Vimentin, Vim |
| Calculated MW | 53733 Da |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 1.5-2.5 µg/ml, Human, rabbit, pig, rat, By Heat Western blot, 0.5-1 µg/ml, Human, rabbit, pig, rat |
| Subcellular Localization | Nucleus matrix . Cytoplasm . |
| Tissue Specificity | VIM: Highly expressed in fibroblasts, someexpression in T- and B-lymphocytes, and little or no expression inBurkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
| Source | Eukaryota |
| Protein Name | Vimentin |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | V9 |
| Immunogen | Pig eye lens vimentin. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the intermediate filament family. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Vim {ECO:0000312|RGD:621646} |
|---|---|
| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP- mediated recruitment to outgrowth tips (By similarity). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:P08670}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P08670}. Nucleus matrix. Cell membrane {ECO:0000250|UniProtKB:P20152}. Cell projection, axon {ECO:0000250|UniProtKB:P20152} |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The VIM gene was one of many that Gieser and Swaroop(1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10.
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