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Anti-Alpha-Actinin Antibody (Monoclonal, EA-53)

Figure 1. Western blot analysis of Alpha-Actinin using anti- Alpha-Actinin antibody (ABO10487). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat cardiac muscle tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse cardiac muscle tissue lysates,Lane 4: mouse skeletal muscle tissue lysates,Lane 5: human placenta tissue lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Alpha-Actinin antigen affinity purified monoclonal antibody (Catalog # ABO10487) at 0.5 Î¼g/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Alpha-Actinin at approximately 103KD. The expected band size for Alpha-Actinin is at 103KD.
Figure 2. IHC analysis of Alpha-Actinin using anti- Alpha-Actinin antibody (ABO10487).Alpha-Actinin was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1.5Î¼g/ml mouse anti- Alpha-Actinin Antibody (ABO10487) overnight at 4Â°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Figure 3. IHC analysis of Alpha-Actinin using anti- Alpha-Actinin antibody (ABO10487).Alpha-Actinin was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1.5Î¼g/ml mouse anti- Alpha-Actinin Antibody (ABO10487) overnight at 4Â°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F
Primary Accession	D3ZCV0
Host	Mouse
Isotype	Mouse IgG1
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Format	Lyophilized
Description	Mouse IgG monoclonal antibody for alpha-Actinin, actinin, alpha 2 (ACTN2 ) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat;rabbit. No cross reactivity with other proteins.
Reconstitution	Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Additional Information
Calculated MW	103834 Da
Application Details	Immunohistochemistry(Paraffin-embedded Section), 1-2 µg/ml, Human, mouse, rat, rabbit, By Heat Immunohistochemistry(Frozen Section), 1-2 µg/ml, Human, mouse, rat, rabbit, - Western blot, 0.5-1 µg/ml, Human, mouse, rat, rabbit
Subcellular Localization	ACTN2: Cytoplasm, myofibril, sarcomere, Z line\|Actn2: Cytoplasm, myofibril, sarcomere, Z line
Tissue Specificity	ACTN2: Expressed in both skeletal and cardiac muscle.
Protein Name	Alpha-actinin-2
Contents	Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clone Names	EA-53
Immunogen	Rabbit skeletal alpha-actinin.
Purification	Ascites
Cross Reactivity	No cross reactivity with other proteins
Storage	At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities	Belongs to the alpha-actinin family.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin(alpha A) shares structural homology with spectrin and dystrophin.

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¥ 3,367.00

Cat# ABO10487

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100 µg

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Anti-Alpha-Actinin Antibody (Monoclonal, EA-53)

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