癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-Alpha-Adaptin Antibody (Monoclonal, 100/2)
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- 背景知识
Application 
| WB |
|---|---|
| Primary Accession | D3ZUY8 |
| Host | Mouse |
| Isotype | Mouse IgG2a |
| Reactivity | Human, Rat |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for alpha-Adaptin, adaptor-related protein complex 2, alpha 1 subunit (AP2A1 ) detection. Tested with WB in Human;rat. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Calculated MW | 107675 Da |
|---|---|
| Application Details | Western blot, 2-4 µg/ml, Human, rat |
| Subcellular Localization | AP2A1: Cell membrane Membrane,coated pit Note: AP-2 appearsto be excluded from internalizing CCVs and to disengage from sitesof endocytosis seconds before internalization of the nascent CCV.|Ap2a1: Cell membrane Membrane,coated pit Note: AP-2 appearsto be excluded from internalizing CCVs and to disengage from sitesof endocytosis seconds before internalization of the nascent CCV. |
| Tissue Specificity | AP2A1: Isoform A expressed in forebrain, skeletalmuscle, spinal cord, cerebellum, salivary gland, heart and colon.Isoform B is widely expressed in tissues and also in breast cancerand in prostate carcinoma cells. |
| Protein Name | AP-2 complex subunit alpha-1 |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | 100/2 |
| Immunogen | AP-2 adaptor polypeptides from bovine brain. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the adaptor complexes large subunit family. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1(HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al.(2001) identified 3 HIP1-associated proteins, clathrin heavy chain(CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD(adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.
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