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Anti-Connexin 43/GJA1 Antibody
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- 背景知识
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | P17302 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Gap junction alpha-1 protein( GJA1) detection. Tested with WB, IHC-P in Human;Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 2697 |
|---|---|
| Other Names | Gap junction alpha-1 protein, Connexin-43, Cx43, Gap junction 43 kDa heart protein, GJA1, GJAL |
| Calculated MW | 43008 Da |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat |
| Subcellular Localization | Cell membrane ; Multi-pass membrane protein . Cell junction, gap junction . Endoplasmic reticulum . |
| Tissue Specificity | Expressed in the heart and fetal cochlea. . |
| Source | Eukaryota |
| Protein Name | Gap junction alpha-1 protein |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 43(366-382aa RASSRASSRPRPDDLEI), identical to the related rat and mouse sequences. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the connexin family. Alpha-type (group II) subfamily. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | GJA1 |
|---|---|
| Synonyms | GJAL |
| Function | Structural component of the gap junction, a specialized intercellular structure consisting of a cluster of closely packed pairs of transmembrane channels, the connexons, that allow passage of small molecules and electrical signals between neighboring cells (By similarity). Forms homotypic and heterotypic channels gated by transjunctional voltage (By similarity). May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph (Probable). Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in the conductive system of ventricular myocardium and heart morphogenesis (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization (By similarity). Involved in intercellular innate immune signaling (PubMed:24077100, PubMed:31992625, PubMed:40010341). Mediates translocation of 2',3'-cGAMP and 2',5'-oligoadenylates (2-5A) second messengers from virus-infected cells to macrophages and uninfected neighboring cells to propagate and amplify the antiviral immune response (PubMed:24077100, PubMed:31992625, PubMed:40010341). |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. Endoplasmic reticulum {ECO:0000250|UniProtKB:P23242}. Cell junction. Note=Localizes at the intercalated disk (ICD) between two adjacent cardiomyocytes and the proper localization at ICD is dependent on TMEM65. {ECO:0000250|UniProtKB:P23242} |
| Tissue Location | Expressed at intercalated disks in the heart (at protein level) (PubMed:11741837, PubMed:18662195, PubMed:38375917) Expressed in the fetal cochlea (PubMed:11741837). Expressed by keratinocytes in the skin (at protein level) (PubMed:26604139) |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Connexins 43(Cx43), also called GAP Junction Protein, alpha-1(GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.
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