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Anti-PUMA Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB |
|---|---|
| Primary Accession | Q9BXH1 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Bcl-2-binding component 3(BBC3) detection. Tested with WB in Human;Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 27113 |
|---|---|
| Other Names | Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis, BBC3, PUMA |
| Calculated MW | 20532 Da |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse |
| Subcellular Localization | Mitochondrion . Localized to the mitochondria in order to induce cytochrome c release. |
| Tissue Specificity | Ubiquitously expressed. . |
| Source | Eukaryota |
| Protein Name | Bcl-2-binding component 3 |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PUMA(145-159aa ADDLNAQYERRRQEE), identical to the related rat and mouse sequences. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the Bcl-2 family. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | BBC3 |
|---|---|
| Synonyms | PUMA |
| Function | Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis (PubMed:11463391, PubMed:23340338). Promotes partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53, releasing the bound p53/TP53 to induce apoptosis (PubMed:23340338). Regulates ER stress-induced neuronal apoptosis (By similarity). |
| Cellular Location | Mitochondrion Note=Localized to the mitochondria in order to induce cytochrome c release |
| Tissue Location | Ubiquitously expressed. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91% amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.
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