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Anti-DCI Antibody
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Application 
| WB, IHC-P, ICC |
|---|---|
| Primary Accession | P42126 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Enoyl-CoA delta isomerase 1, mitochondrial(ECI1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 1632 |
|---|---|
| Other Names | Enoyl-CoA delta isomerase 1, mitochondrial, 5.3.3.8, 3, 2-trans-enoyl-CoA isomerase, Delta(3), Delta(2)-enoyl-CoA isomerase, D3, D2-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, ECI1, DCI |
| Calculated MW | 32816 Da |
| Application Details | Immunocytochemistry , 0.5-1 µg/ml, Human, - Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse |
| Subcellular Localization | Mitochondrion matrix. |
| Source | Eukaryota |
| Protein Name | Enoyl-CoA delta isomerase 1, mitochondrial |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human DCI(272-290aa ADVQNFVSFISKDSIQKSL), different from the related mouse sequence by two amino acids and from the related rat sequence by three amino acids. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | ECI1 |
|---|---|
| Synonyms | DCI |
| Function | Auxiliary enzyme in the beta-oxidation of mono- and polyunsaturated fatty acids (PubMed:26474213, PubMed:7818490). Together with the 2,4-dienoyl-CoA reductase (DECR) and the Delta(3,5)- Delta(2,4)-dienoyl-CoA isomerase (ECH1), they allow the reentrance of the enoyl-CoA into the beta-oxidation cycle (PubMed:26474213). Able to isomerize both 3-cis (3Z) and 3-trans (3E) double bonds into the 2- trans (2E) form in a range of fatty enoyl-CoA species, with a preference for (3Z)-enoyl-CoAs over (3E)-enoyl-CoAs (PubMed:26474213, PubMed:7818490). The catalytic efficiency of this enzyme is not affected by the fatty acyl chain length, and based on its established catalytic mechanism, it is predicted to act also on other fatty acids besides those tested experimentally (By similarity). |
| Cellular Location | Mitochondrion matrix {ECO:0000250|UniProtKB:P23965} |
| Tissue Location | Expressed in liver (at protein level). |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ECI1/DCI(Dodecenoyl-CoA Delta Isomerase), also known as 3,2-trans-enoyl-CoA isomerase, is an enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon(position 3) to trans double bonds at beta-carbon(position 2). It plays a particularly important role in the metabolism of unsaturated fatty acids. All classes of enoyl-CoA isomerases belong to a family of enzymes, the hydratase/isomerase or crotonase superfamily, and when examined with x-ray crystallography, exhibit a common structural feature of the family, the N-terminal core with a spiral fold composed of four turns, each turn consisting of two beta-sheets and one alpha-helix. Dodecenoyl-CoA Delta Isomerase is involved in the beta-oxidation, one of the most frequently used pathways in fatty acid degradation, of unsaturated fatty acids with double bonds at odd-numbered carbon positions. It does so by shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or trans-enoyl-CoA to 2-trans-enoyl-CoA.
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