癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-PTCH2 Antibody
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Application 
| WB |
|---|---|
| Primary Accession | Q9Y6C5 |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Protein patched homolog 2(PTCH2) detection. Tested with WB in Human;Mouse. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 8643 |
|---|---|
| Other Names | Protein patched homolog 2, PTC2, PTCH2 |
| Calculated MW | 130544 Da |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human, Mouse |
| Subcellular Localization | Membrane; Multi-pass membrane protein. |
| Source | Eukaryota |
| Protein Name | Protein patched homolog 2(PTC2) |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PTCH2(885-901aa WLHDKYDTTGENLRIPP), different from the related mouse sequence by one amino acid. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the patched family. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | PTCH2 {ECO:0000303|PubMed:9811851, ECO:0000312|HGNC:HGNC:9586} |
|---|---|
| Function | Negative regulator of the smoothened signaling pathway, which acts as a receptor for hedgehog (SHH, IHH and DHH) morphogens (PubMed:9811851). In absence of hedgehog, acts as an inhibitor of smoothened protein (SMO) by preventing SMO access to cholesterol: mechanistically, acts as a cholesterol transporter, which depeletes cholesterol from the outer leaflet of plasma membrane, thereby preventing cholesterol-binding to the extracellular CRD region of SMO. Hedgehog-binding inhibits PTCH1 cholesterol transporter activity, promoting SMO activation by cholesterol and transduction of the smoothened signaling pathway. Molecular mechanism governing cholesterol transport are still unclear: may move cholesterol from the outer to the inner leaflet of the membrane in exchange for potassium or sodium ion export in the opposite direction (By similarity). In contrast to PTCH1 ortholog, PTCH2 is not essential for viability and plays a more limited role. Plays a role in epidermal homeostasis in mature skin (By similarity). Plays a role in the control of cellular growth (PubMed:18285427). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:Q61115}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q61115}. Cell projection, cilium membrane {ECO:0000250|UniProtKB:Q61115}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q61115}. Note=Localizes to primary cilium and prevents cilium localization of SMO. Delocalizes from primary cilium following hedgehog-binding {ECO:0000250|UniProtKB:Q61115} |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
PTCH2(patched 2), also known as PTC2, encodes a 1.203-amino acid putative transmembrane protein that is highly homologous to the PTCH product. The Patched-hedgehog signaling pathway is involved in establishing segment polarity and the anterior-posterior orientation of a number of developing appendages. The pathway is conserved from fly to vertebrates and has been implicated in a number of human neoplasms and developmental syndromes. Mutations in the human Patched gene are the basis of the nevoid basal cell carcinoma syndrome. With age, homozygous mutant male mice developed skin lesions consisting of alopecia and epidermal hyperplasia, suggesting a role for Ptch2 in adult epidermal homeostasis via Shh signaling.
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