癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-NADPH Oxidase 4 Antibody
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Application 
| WB, IHC-P |
|---|---|
| Primary Accession | Q9JHI8 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for NADPH oxidase 4(NOX4) detection. Tested with WB, IHC-P in Human;Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 50490 |
|---|---|
| Other Names | NADPH oxidase 4, 1.6.3.-, Kidney oxidase-1, KOX-1, Kidney superoxide-producing NADPH oxidase, Renal NAD(P)H-oxidase, Superoxide-generating NADPH oxidase 4, Nox4, Renox |
| Calculated MW | 66519 Da |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Rat, Human, Mouse, By Heat Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse |
| Subcellular Localization | Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell junction, focal adhesion . Cell membrane . May localize to plasma membrane and focal adhesions. . |
| Tissue Specificity | EXpressed in brain, in all layers of the cerebellum, in pyramidal cells of the Ammon horn and in Purkinje cells (at protein level). Expressed in osteoclasts, leukocytes, kidney, liver and lung. . |
| Source | Eukaryota |
| Protein Name | NADPH oxidase 4 |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse NADPH oxidase 4(561-578aa NRNNSYGTKFEYNKES), identical to the related rat sequence and different from the related human sequence by two amino acids. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Contains 1 FAD-binding FR-type domain. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Nox4 |
|---|---|
| Synonyms | Renox |
| Function | NADPH oxidase that catalyzes predominantly the reduction of oxygen to H2O2 (By similarity). Can also catalyze to a smaller extent, the reduction of oxygen to superoxide (PubMed:10869423, PubMed:11098048, PubMed:15638999). May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity (By similarity). May regulate insulin signaling cascade (By similarity). May play a role in apoptosis, bone resorption and lipolysaccharide- mediated activation of NFKB (By similarity). May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation (By similarity). Promotes ferroptosis, reactive oxygen species production and reduced glutathione (GSH) levels by activating NLRP3 inflammasome activation and cytokine release (By similarity). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q9NPH5}. Endoplasmic reticulum membrane; Multi- pass membrane protein. Cell membrane {ECO:0000250|UniProtKB:Q9NPH5}; Multi-pass membrane protein. Cell junction, focal adhesion {ECO:0000250|UniProtKB:Q924V1}. Nucleus {ECO:0000250|UniProtKB:Q9NPH5} |
| Tissue Location | EXpressed in brain, in all layers of the cerebellum, in pyramidal cells of the Ammon horn and in Purkinje cells (at protein level). Expressed in osteoclasts, leukocytes, kidney, liver and lung. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
NOX4(NADPH oxidase 4), also called RENAL NADPH OXIDASE or RENOX, is an enzyme that in humans is encoded by the NOX4 gene, and a member of the NOX family of NADPH oxidases. Geiszt et al.(2000) stated that the nucleotide sequence of RENOX matches that found in a genomic clone on chromosome 15. In a note added in proof, they stated that genomic clones assigned to chromosome 11 also contain sequence corresponding to RENOX. By FISH, Shiose et al.(2001) mapped the NOX4 gene to chromosome 11q14.2-q21. Geiszt et al.(2000) found that NIH 3T3 fibroblasts overexpressing transfected RENOX showed increased production of superoxide and developed signs of cellular senescence. They suggested that RENOX, as a renal source of ROS, may fulfill the function of the putative oxygen sensor in the kidney. By biochemical analysis of endogenous renal NOX4, Shiose et al.(2001) determined that the enzyme can use either NADH or NADPH as an electron donor for superoxide production.
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