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Anti-ProProtein Convertase PC4 Antibody
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Application 
| WB |
|---|---|
| Primary Accession | P29121 |
| Host | Rabbit |
| Reactivity | Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Proprotein convertase subtilisin/kexin type 4(PCSK4) detection. Tested with WB in Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 18551 |
|---|---|
| Other Names | Proprotein convertase subtilisin/kexin type 4, 3.4.21.-, KEX2-like endoprotease 3, Neuroendocrine convertase 3, NEC 3, Prohormone convertase 3, Pcsk4, Nec-3, Nec3 |
| Calculated MW | 73214 Da |
| Application Details | Western blot, 0.1-0.5 µg/ml, Rat, Mouse |
| Tissue Specificity | Expressed abundantly in the testis. . |
| Source | Eukaryota |
| Protein Name | Proprotein convertase subtilisin/kexin type 4 |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse Proprotein convertase PC4(117-133aa DPWFSKQWYMNKEIQQD), different from the related rat sequence by one amino acid. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the peptidase S8 family. Furin subfamily. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Pcsk4 |
|---|---|
| Synonyms | Nec-3, Nec3 |
| Function | Proprotein convertase involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. In males, important for ADAM2 processing as well as other acrosomal proteins with roles in fertilization and critical for normal fertilization events such as sperm capacitation, acrosome reaction and binding of sperm to zona pellucida (PubMed:16371590, PubMed:19342015, PubMed:21302280, PubMed:9192653). Also plays a role in female fertility, involved in the regulation of trophoblast migration and placental development, may be through the proteolytical processing and activation of proteins such as IGF2 (By similarity). May also participate in folliculogenesis in the ovaries (PubMed:11164898). |
| Cellular Location | Cytoplasmic vesicle, secretory vesicle, acrosome membrane |
| Tissue Location | Expressed abundantly in the testis since postnatal Day 16 (PubMed:1372895, PubMed:16371590, PubMed:21302280). In testis, strongly detected in round and elongated spermatids as well as spermatocytes. Also observed in residual bodies engulfed by Sertoli cells at spermatogenic stages VIII and IX (PubMed:16371590). In ovaries, expressed in macrophage-like cells of the ovarian theca, interstitium and corpora lutea (PubMed:11164898) |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
PCSK4(Proprotein Convertase, Subtilisin/Kexin-Type, 4), is an enzyme that in humans is encoded by the PCSK4 gene. The gene for PC4(Pcsk4 locus) was mapped to mouse chromosome 10, close to the adipsin locus and near the gene encoding anti-mullerian hormone. Qiu et al.(2005) found that aberrant processing of pro-IGF2 by PC4 may be a cause of IUGR. PC4 cleaved pro-IGF2 to generate the intermediate processed form, IGF2(1-102). Mature IGF2(1-67), likely resulting from further processing by carboxypeptidases, is capable of activating invasive trophoblast cells through AKT phosphorylation. Inhibition of PC4 by a PC4-specific inhibitor blocked pro-IGF2 processing and reduced trophoblast cell migration.
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