癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-CNTF Picoband Antibody
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Application 
| WB, IHC-P |
|---|---|
| Primary Accession | P51642 |
| Host | Rabbit |
| Reactivity | Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Ciliary neurotrophic factor(CNTF) detection. Tested with WB, IHC-P in Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 12803 |
|---|---|
| Other Names | Ciliary neurotrophic factor, CNTF, Cntf |
| Calculated MW | 22587 Da |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Mouse, Rat, By Heat Western blot, 0.1-0.5 µg/ml, Mouse |
| Subcellular Localization | Cytoplasm. |
| Tissue Specificity | Nervous system. |
| Source | Eukaryota |
| Protein Name | Ciliary neurotrophic factor |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Immunogen | E.coli-derived mouse CNTF recombinant protein (Position: A2-M198). Mouse CNTF shares 83% and 95% amino acid (aa) sequences identity with human and rat CNTF, respectively. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Cntf {ECO:0000312|MGI:MGI:88439} |
|---|---|
| Function | Functions as a cytokine through the ciliary neurotrophic factor receptor (CNTFR) complex. The CNTFR complex indeed consists of the membrane-anchored non-signaling receptor CNTFR and the two signaling receptor subunits IL6ST/gp130 and LIFR that transduce the signal into the cell. Functionally, promotes neuronal survival, supports differentiation of various neuronal and non-neuronal cells, and importantly regulates oligodendrocyte progenitor proliferation. Mechanistically, ligand binding to the CNTFR induces dimerization of the IL6ST/gp130 and LIFR, which activates JAK tyrosine kinases (JAK1 or JAK2 and to lesser extent TYK2) bound to their intracellular domains. These kinases subsequently phosphorylate IL6ST/gp130 and LIFR. The tyrosine phosphorylated signaling receptors serve in turn as docking sites for recruitment and activation of signal transducer and activators of transcription (STAT3 and to lesser extent STAT1). Can also signal via trans-signaling using soluble forms of CNTFR (By similarity). Seems to prevent the degeneration of motor axons after axotomy (By similarity). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:P26441}. Secreted {ECO:0000250|UniProtKB:Q02011}. Note=The precursor semms to be cytoplasmic (By similarity). The lack of a specific hydrophobic segment in the precursor sequence suggests that CNTF is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins (By similarity). {ECO:0000250|UniProtKB:P20294, ECO:0000250|UniProtKB:P26441} |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Ciliary neurotrophic factor (CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The mouse CNTF gene is on mouse chromosome 19 and that its expression is unaffected in the mouse neurologic mutant wobbler, a form of spinal muscular atrophy. The CNTF protein is highly conserved in evolution. The protein is a potent survival factor for neurons and oligodendrocytes, and it may be involved in reducing tissue destruction during inflammatory attacks. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.
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