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Anti-SP-D Antibody

Anti- Surfactant protein D Picoband antibody, ABO12303, Western blottingAll lanes: Anti Surfactant protein D (ABO12303) at 0.5ug/mlLane 1: Rat Lung Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: PANC Whole Cell Lysate at 40ugPredicted bind size: 38KDObserved bind size: 38KD
Anti- Surfactant protein D Picoband antibody, ABO12303, IHC(P)IHC(P): Human Lung Cancer Tissue
Figure 3. IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (ABO12303).Surfactant protein D was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1Î¼g/ml rabbit anti-Surfactant protein D Antibody (ABO12303) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Figure 4. IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (ABO12303).Surfactant protein D was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1Î¼g/ml rabbit anti-Surfactant protein D Antibody (ABO12303) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Figure 5. IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (ABO12303).Surfactant protein D was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1Î¼g/ml rabbit anti-Surfactant protein D Antibody (ABO12303) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. 5

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, ICC, E
Primary Accession	P35247
Host	Rabbit
Reactivity	Human, Mouse, Rat
Clonality	Polyclonal
Format	Lyophilized
Description	Rabbit IgG polyclonal antibody for Pulmonary surfactant-associated protein D(SFTPD) detection. Tested with WB, IHC-P, IHC-F, ICC, ELISA in Human;Mouse; Rat.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Additional Information
Gene ID	6441
Other Names	Pulmonary surfactant-associated protein D, PSP-D, SP-D, Collectin-7, Lung surfactant protein D, SFTPD, COLEC7, PSPD, SFTP4
Calculated MW	37728 Da
Application Details	ELISA , 0.1-0.5 µg/ml Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, By Heat Immunohistochemistry(Frozen Section), 0.5-1 µg/ml Immunocytochemistry, 0.5-1 µg/ml Western blot, 0.1-0.5 µg/ml
Subcellular Localization	Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
Tissue Specificity	Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes). .
Source	Eukaryota
Protein Name	Pulmonary surfactant-associated protein D
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human Surfactant protein D (292-321aa RSAAENAALQQLVVAKNEAAFLSMTD SKTE), different from the related mouse and rat sequences by eight amino acids.
Purification	Immunogen affinity purified.
Cross Reactivity	No cross reactivity with other proteins
Storage	At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities	Belongs to the SFTPD family.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SFTPD
Synonyms	COLEC7, PSPD, SFTP4
Function	Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha- glucosyl moieties.
Cellular Location	Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film
Tissue Location	Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes).

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. It is mapped to 10q22.2-q23.1. The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism.

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¥ 3,367.00

Cat# ABO12303

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100 µg

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Anti-SP-D Antibody

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