癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-KIAA1524 Picoband Antibody
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Application 
| WB |
|---|---|
| Primary Accession | Q8TCG1 |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Protein CIP2A(KIAA1524) detection. Tested with WB in Human;Mouse. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 57650 |
|---|---|
| Other Names | Protein CIP2A, Cancerous inhibitor of PP2A, p90 autoantigen, KIAA1524, CIP2A |
| Calculated MW | 102185 Da |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human, Mouse |
| Subcellular Localization | Membrane ; Single-pass membrane protein . Cytoplasm . Slightly concentrates in the perinuclear region. |
| Tissue Specificity | Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level). . |
| Source | Eukaryota |
| Protein Name | Protein CIP2A |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human KIAA1524 (854-889aa EVQKAQLEGRLEEKESLVKLQQEELNKHSHMIAMIH), different from the related mouse sequence by three amino acids. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | CIP2A {ECO:0000303|PubMed:17632056, ECO:0000312|HGNC:HGNC:29302} |
|---|---|
| Function | Acts as an inhibitor of protein phosphatase PP2A (PubMed:17632056). Promotes anchorage-independent cell growth and tumor formation by preventing dephosphorylation of MYC, thereby stabilizing MYC in human malignancies (PubMed:17632056). Together with TOPBP1, plays an essential role in the response to genome instability generated by the presence of acentric chromosome fragments derived from shattered chromosomes within micronuclei (PubMed:35121901, PubMed:35842428, PubMed:37165191, PubMed:37316668). Micronuclei, which are frequently found in cancer cells, consist of chromatin surrounded by their own nuclear membrane: following breakdown of the micronuclear envelope, a process associated with chromothripsis, the CIP2A-TOPBP1 complex tethers chromosome fragments during mitosis to ensure clustered segregation of the fragments to a single daughter cell nucleus, facilitating re-ligation with limited chromosome scattering and loss (PubMed:37165191, PubMed:37316668). |
| Cellular Location | Cytoplasm. Chromosome. Note=Predominantly localizes within the cytoplasm (PubMed:35842428). Localizes to broken chromosomes within micronuclei during interphase and following chromothripsis (PubMed:37165191, PubMed:37316668). Localization to broken chromosomes is mainly independent of MDC1 (PubMed:35121901, PubMed:37165191) |
| Tissue Location | Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC) Present in liver cancer cells (at protein level) |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Protein CIP2A, also known as cancerous inhibitor of PP2A (CIP2A), is a protein that in humans is encoded by the KIAA1524 gene. CIP2A is required for the malignant cellular growth and for in vivo tumor formation. In accordance with the oncogenic role of CIP2A, overexpression of CIP2A promotes Ras-elicited cell growth and transforms immortalized human cells (HEK-TERVs). More recently CIP2A has been shown to regulate phosphorylation and activity of many other oncoproteins and to drive malignant cell growth and tumorigenesis in various human cancer types. Importantly, CIP2A deficient mice are viable, suggesting that targeting of oncogenic function of CIP2A would not result in serious side-effects.
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