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Anti-FASN Picoband Antibody

Western blot analysis of FASN expression in HEPG2 whole cell lysates (lane 1), U87 whole cell lysates (lane 2) and RAJI whole cell lysates (lane 3). FASN at 273KD was detected using rabbit anti- FASN Antigen Affinity purified polyclonal antibody (Catalog # ABO12551) at0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
FASN was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- FASN Antigen Affinity purified polyclonal antibody (Catalog # ABO12551) at 1 Î¼g/mL. The immunohistochemical section was developed using SABC method (Catalog # SA2010).
Figure 3. IHC analysis of FASN using anti-FASN antibody (ABO12551).FASN was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1Î¼g/ml rabbit anti-FASN Antibody (ABO12551) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Figure 4. IHC analysis of FASN using anti-FASN antibody (ABO12551).FASN was detected in immunocytochemical section of MCF-7 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1Î¼g/ml rabbit anti-FASN Antibody (ABO12551) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, ICC
Primary Accession	P49327
Host	Rabbit
Reactivity	Human, Mouse, Rat
Clonality	Polyclonal
Format	Lyophilized
Description	Rabbit IgG polyclonal antibody for Fatty acid synthase(FASN) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Additional Information
Gene ID	2194
Other Names	Fatty acid synthase, 2.3.1.85, [Acyl-carrier-protein] S-acetyltransferase, 2.3.1.38, [Acyl-carrier-protein] S-malonyltransferase, 2.3.1.39, 3-oxoacyl-[acyl-carrier-protein] synthase, 2.3.1.41, 3-oxoacyl-[acyl-carrier-protein] reductase, 1.1.1.100, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, 4.2.1.59, Enoyl-[acyl-carrier-protein] reductase, 1.3.1.39, Oleoyl-[acyl-carrier-protein] hydrolase, 3.1.2.14, FASN, FAS
Calculated MW	273427 Da
Application Details	Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, By Heat Immunocytochemistry,0.5-1 µg/ml Western blot, 0.1-0.5 µg/ml
Subcellular Localization	Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tissue Specificity	Ubiquitous. Prominent expression in brain, lung, and liver. .
Source	Eukaryota
Protein Name	Fatty acid synthase
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen	E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively.
Purification	Immunogen affinity purified.
Cross Reactivity	No cross reactivity with other proteins.
Storage	At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	FASN
Synonyms	FAS
Function	Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.
Cellular Location	Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Tissue Location	Ubiquitous. Prominent expression in brain, lung, liver and mammary gland.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Fatty acid synthaseÂ (FAS) is anÂ enzymeÂ that in humans is encoded by theÂ FASNÂ gene. It is mapped to 17q25. The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

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¥ 3,367.00

Cat# ABO12551

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100 µg

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¥ 3,367.00

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Anti-FASN Picoband Antibody

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