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Anti-human DDT Antibody
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- 背景知识
Application 
| WB, IHC-P, ICC, E |
|---|---|
| Primary Accession | P30046 |
| Host | Rabbit |
| Reactivity | Human |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for D-dopachrome decarboxylase(DDT) detection. Tested with WB, IHC-P, ICC, ELISA in Human. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 1652 |
|---|---|
| Other Names | D-dopachrome decarboxylase, 4.1.1.84, D-dopachrome tautomerase, Phenylpyruvate tautomerase II, DDT |
| Calculated MW | 12712 Da |
| Application Details | ELISA , 0.1-0.5 µg/ml, Human, - Immunocytochemistry , 0.5-1 µg/ml, Human, - Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat Western blot, 0.1-0.5 µg/ml, Human |
| Subcellular Localization | Cytoplasm . |
| Source | Eukaryota |
| Protein Name | D-dopachrome decarboxylase |
| Contents | Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify Carrier Free" or "No BSA" in your order note. " |
| Immunogen | E. coli-derived human DDT recombinant protein(Position: M1-L118). |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | DDT |
|---|---|
| Function | Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI). |
| Cellular Location | Cytoplasm. |
| Tissue Location | Highly expressed in the liver and at lower levels in the heart, lung and pancreas. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10.
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