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Anti-CD252 Picoband Antibody

Figure 1. Western blot analysis of CD252 using anti-CD252 antibody (ABO12986). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse CD252 protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD252 antigen affinity purified polyclonal antibody (Catalog # ABO12986) at 0.5 Î¼g/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD252 at approximately 21-25KD. The expected band size for CD252 is at 18KD.
Figure 2. IHC analysis of CD252 using anti-CD252 antibody (ABO12986).CD252 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1Î¼g/ml rabbit anti-CD252 Antibody (ABO12986) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Primary Accession	P43488
Host	Rabbit
Reactivity	Mouse
Clonality	Polyclonal
Format	Lyophilized
Description	Rabbit IgG polyclonal antibody for CD252 detection. Tested with WB, ELISA(Cap) in Mouse.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Additional Information
Gene ID	22164
Other Names	Tumor necrosis factor ligand superfamily member 4, OX40 ligand, OX40L, CD252, Tnfsf4, Ox40l, Txgp1l
Calculated MW	22255 Da
Application Details	Western blot, 0.1-0.5 µg/ml ELISA(Cap), 0.1-0.5 µg/ml
Subcellular Localization	Membrane; Single-pass type II membrane protein.
Source	Eukaryota
Contents	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.
Immunogen	E. coli-derived mouse CD252 recombinant protein (Position: Q49-L198).
Cross Reactivity	No cross reactivity with other proteins.
Storage	At -20˚C; for one year. After r˚Constitution, at 4˚C; for one month. It˚Can also be aliquotted and stored frozen at -20˚C; for a longer time. Avoid repeated freezing and thawing.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	Tnfsf4
Synonyms	Ox40l, Txgp1l
Function	Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
Cellular Location	Membrane; Single-pass type II membrane protein.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

OX40L(TNFSF4) is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.

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¥ 3,367.00

Cat# ABO12986

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100 µg

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¥ 3,367.00

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Anti-CD252 Picoband Antibody

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