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Anti-HE4 Picoband Antibody

Figure 1. Western blot analysis of HE4 using anti-HE4 antibody (ABO12992). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat brain tissue lysates,Lane 3: rat thymus tissue lysates,Lane 4: mouse testis tissue lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse thymus tissue lysates,Lane 7: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HE4 antigen affinity purified polyclonal antibody (Catalog # ABO12992) at 0.5 ug/mL overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HE4 at approximately 19KD. The expected band size for HE4 is at 13KD.
Figure 2. IHC analysis of HE4 using anti-HE4 antibody (ABO12992).HE4 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HE4 Antibody (ABO12992) overnight at 4â„ƒ. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37â„ƒ. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Figure 3. IHC analysis of HE4 using anti-HE4 antibody (ABO12992).HE4 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HE4 Antibody (ABO12992) overnight at 4â„ƒ. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37â„ƒ. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Figure 4. IHC analysis of HE4 using anti-HE4 antibody (ABO12992).HE4 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HE4 Antibody (ABO12992) overnight at 4â„ƒ. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37â„ƒ. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Figure 5. IHC analysis of HE4 using anti-HE4 antibody (ABO12992).HE4 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HE4 Antibody (ABO12992) overnight at 4â„ƒ. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37â„ƒ. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Figure 6. IHC analysis of HE4 using anti-HE4 antibody (ABO12992).HE4 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HE4 Antibody (ABO12992) overnight at 4â„ƒ. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37â„ƒ. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Wfdc2: Q8CHN3
Host	Rabbit
Reactivity	Mouse, Rat
Clonality	Polyclonal
Format	Lyophilized
Description	Rabbit IgG polyclonal antibody for HE4 detection. Tested with WB, IHC-P, ELISA(Cap) in Mouse;Rat.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Additional Information
Application Details	Western blot, 0.1-0.5 µg/ml Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml ELISA(Cap), 0.1-0.5 µg/ml
Subcellular Localization	Secreted .
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen	E. coli-derived rat HE4 recombinant protein (Position: E31-F168).
Purification	Immunogen affinity purified.
Cross Reactivity	No cross reactivity with other proteins.
Storage	At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

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¥ 3,367.00

Cat# ABO12992

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100 µg

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¥ 3,367.00

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Anti-HE4 Picoband Antibody

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