Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated

Western blot analysis of beta Tubulin expression in (1) Jurkat cell lysate, (2) Human kidney lysate, (3) 3T3 cell lysate, (4) Mouse brain lysate, (5) C6 cell lysate, (6) Rat heart lysate (H01857).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB3 monoclonal antibody (Catalog # H01857) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBB3

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	Q13509
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated . Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Additional Information
Gene ID	10381
Other Names	Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB3, TUBB4
Calculated MW	50433 Da
Application Details	WB 1:5000-1:20000
Subcellular Localization	Cytoplasm, cytoskeleton.
Tissue Specificity	Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues..
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: DF-20
Immunogen	A synthesized peptide derived from human beta Tubulin
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TUBB3
Synonyms	TUBB4
Function	Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers (PubMed:34996871, PubMed:38305685, PubMed:38609661). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871, PubMed:38305685, PubMed:38609661). Below the cap, alpha-beta tubulin heterodimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871, PubMed:38609661). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).
Cellular Location	Cytoplasm, cytoskeleton. Cell projection, growth cone {ECO:0000250\|UniProtKB:Q9ERD7}. Cell projection, lamellipodium {ECO:0000250\|UniProtKB:Q9ERD7}. Cell projection, filopodium {ECO:0000250\|UniProtKB:Q9ERD7}
Tissue Location	Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.