Anti-Phospho-Stat5 (Y694) STAT5A Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human thyroid, using Phospho-Stat5 (Y694) Antibody.
Figure 1. Western blot analysis of STAT5A using anti-STAT5A antibody (P01087-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A antigen affinity purified monoclonal antibody (Catalog # P01087-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5A at approximately 95 kDa. The expected band size for STAT5A is at 91 kDa.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P42229
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-Phospho-Stat5 (Y694) STAT5A Rabbit Monoclonal Antibody . Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Additional Information
Gene ID	6776
Other Names	Signal transducer and activator of transcription 5A, STAT5A, STAT5
Calculated MW	90647 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200
Subcellular Localization	Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: EDD-19
Immunogen	A synthesized peptide derived from human Phospho-Stat5 (Y694)
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	STAT5A
Synonyms	STAT5
Function	Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL- induced transcription. Regulates the expression of milk proteins during lactation.
Cellular Location	Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.