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>   首页   >   产品   >   一抗   >   信号转导   >   Anti-CCR9 Rabbit Monoclonal Antibody   

Anti-CCR9 Rabbit Monoclonal Antibody

     
  • 1 - Anti-CCR9 Rabbit Monoclonal Antibody ABO13297
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
  • 1 - Anti-CCR9 Rabbit Monoclonal Antibody ABO13297
    Figure 1. Western blot analysis of CCR9 using anti-CCR9 antibody (M02348).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human MOLT-4 whole cell lysates,
    Lane 2: human Jurkat whole cell lysates,
    Lane 3: human U-87MG whole cell lysates,
    Lane 4: human K562 whole cell lysates,
    Lane 5: rat thymus tissue lysates,
    Lane 6: rat PC-12 whole cell lysates,
    Lane 7: mouse thymus tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR9 antigen affinity purified monoclonal antibody (Catalog # M02348) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR9 at approximately 42 kDa. The expected band size for CCR9 is at 42 kDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, ICC, FC
Primary Accession P51686
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-CCR9 Rabbit Monoclonal Antibody . Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 10803
Other Names C-C chemokine receptor type 9, C-C CKR-9, CC-CKR-9, CCR-9, G-protein coupled receptor 28, GPR-9-6, CDw199, CCR9, GPR28
Calculated MW 42016 Da
Application Details WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Subcellular Localization Cell membrane; Multi-pass membrane protein.
Tissue Specificity Highly expressed in the thymus and low in lymph nodes and spleen..
Source Eukaryota
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: AAO-3
Immunogen A synthesized peptide derived from human CCR9
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name CCR9
Synonyms GPR28
Function Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level.
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Highly expressed in the thymus and low in lymph nodes and spleen.
Research Areas
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