Anti-BDNF Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:5k dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:50 dilution.
Immunohistochemical analysis of paraffin-embedded Rat pancreas, using the Antibody at 1:50 dilution.
Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:50 dilution.
Immunohistochemical analysis of paraffin-embedded Human lung, using the Antibody at 1:50 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse heart, using the Antibody at 1:50 dilution.
Western blot analysis of extracts of Human cerebellum lysate, using BDNF antibody.
Immunohistochemical analysis of paraffin-embedded human testis, using BDNF Antibody.
Immunofluorescent analysis using the Antibody at 1:500 dilution.
Immunofluorescent analysis of HeLa cells, using BDNF Antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC
Primary Accession	P23560
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-BDNF Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Additional Information
Gene ID	627
Other Names	Brain-derived neurotrophic factor, BDNF, Abrineurin, BDNF precursor form, ProBDNF, BDNF {ECO:0000303\|PubMed:28397838, ECO:0000312\|HGNC:HGNC:1033}
Calculated MW	27818 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200
Subcellular Localization	Secreted.
Tissue Specificity	Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta..
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: BEH-2
Immunogen	A synthesized peptide derived from human BDNF
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	BDNF {ECO:0000303\|PubMed:28397838, ECO:0000312\|HGNC:HGNC:1033}
Function	Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Cellular Location	Secreted
Tissue Location	Detected in blood plasma and in saliva (at protein level) (PubMed:11152678, PubMed:19467646). Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.