Anti-CCR7 Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human spleen, using CCR7 Antibody.
Immunofluorescent analysis of Hela cells, using CCR7 Antibody .
Figure 1. Western blot analysis of CCR7 using anti-CCR7 antibody (M00390).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR7 antigen affinity purified monoclonal antibody (Catalog # M00390) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR7 at approximately 43 kDa. The expected band size for CCR7 is at 43 kDa.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, IP
Primary Accession	P32248
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-CCR7 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Additional Information
Gene ID	1236
Other Names	C-C chemokine receptor type 7, C-C CKR-7, CC-CKR-7, CCR-7, BLR2, CDw197, Epstein-Barr virus-induced G-protein coupled receptor 1, EBI1, EBV-induced G-protein coupled receptor 1, MIP-3 beta receptor, CD197, CCR7, CMKBR7, EBI1, EVI1
Calculated MW	42874 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 IP 1:50
Subcellular Localization	Cell membrane; Multi-pass membrane protein.
Tissue Specificity	Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/ml BSA.
Clone Names	Clone: CFO-3
Immunogen	A synthesized peptide derived from human CCR7
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CCR7
Synonyms	CMKBR7, EBI1, EVI1
Function	Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
Cellular Location	Cell membrane; Multi-pass membrane protein.
Tissue Location	Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.