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>   首页   >   产品   >   一抗   >   信号转导   >   Anti-SOX1 Rabbit Monoclonal Antibody   

Anti-SOX1 Rabbit Monoclonal Antibody

     
  • 1 - Anti-SOX1 Rabbit Monoclonal Antibody ABO13453
    Figure 1. Western blot analysis of SOX1 using anti-SOX1 antibody (M08724).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human K562 whole cell lysates,
    Lane 3: human Jurkat whole cell lysates,
    Lane 4: human Caco-2 whole cell lysates,
    Lane 5: rat brain tissue lysates,
    Lane 6: rat PC-12 whole cell lysates,
    Lane 7: mouse brain tissue lysates,
    Lane 8: mouse Neuro-2a whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SOX1 antigen affinity purified monoclonal antibody (Catalog # M08724) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX1 at approximately 39 kDa. The expected band size for SOX1 is at 39 kDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, IF, ICC
Primary Accession O00570
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-SOX1 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 6656
Other Names Transcription factor SOX-1, SOX1
Calculated MW 39023 Da
Application Details WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Subcellular Localization Nucleus.
Tissue Specificity Mainly expressed in the developing central nervous system.
Source Eukaryota
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: GIA-19
Immunogen A synthesized peptide derived from human SOX1
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name SOX1
Function Transcriptional activator. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
Cellular Location Nucleus.
Tissue Location Mainly expressed in the developing central nervous system
Research Areas
Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol
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¥ 3,367.00
Cat# ABO13453
Size:
100 µl
Price:
¥ 3,367.00
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