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>   首页   >   产品   >   一抗   >   信号转导   >   Anti-SOD2/Mnsod Rabbit Monoclonal Antibody   

Anti-SOD2/Mnsod Rabbit Monoclonal Antibody

     
  • 1 - Anti-SOD2/Mnsod Rabbit Monoclonal Antibody ABO13485
    Figure 1. Western blot analysis of TOMM20/Tom20 using anti-TOMM20/Tom20 antibody (M04039).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human HepG2 whole cell lysates,
    Lane 2: human 293T whole cell lysates,
    Lane 3: human K562 whole cell lysates,
    Lane 4: human Hela whole cell lysates,
    Lane 5: rat kidney tissue lysates,
    Lane 6: rat stomach tissue lysates,
    Lane 7: mouse kidney tissue lysates,
    Lane 8: mouse stomach tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM20/Tom20 antigen affinity purified monoclonal antibody (Catalog # M04039) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOMM20/Tom20 at approximately 16 kDa. The expected band size for TOMM20/Tom20 is at 16 kDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession P04179
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-SOD2/Mnsod Rabbit Monoclonal Antibody . Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 6648
Other Names Superoxide dismutase [Mn], mitochondrial, 1.15.1.1, SOD2
Calculated MW 24750 Da
Application Details WB 1:500-1:2000
IHC 1:50-1:200
Subcellular Localization Mitochondrion matrix.
Source Eukaryota
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: IDO-19
Immunogen A synthesized peptide derived from human SOD2
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name SOD2
Function Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Cellular Location Mitochondrion matrix.
Research Areas
Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol
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¥ 3,367.00
Cat# ABO13485
Size:
100 µl
Price:
¥ 3,367.00
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