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Anti-GAPDH Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded (1) Rat colon; (2) Mouse testis; (3) Human uterus; (4) Human kidney, using GAPDH Antibody.
Western blot analysis of GAPDH expression in (1) Hela cell lysate; (2)Jurkat cell lysate; (3)Mouse kidney lysate; (4) Mouse spleen lysate; (5) RAW 264.7 cell lysate; (6) Rat brain lysate with GAPDH Antibody.
All lanes use GAPDH Antibody at 1:50000 dilution for 1 hour at room temperature.
All lanes use GAPDH Antibody at 1:50000 dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Human pituitary adenoma, using the Antibody at 1:400 dilution.
Immunohistochemical analysis of paraffin-embedded Human squamous carcinoma, using the Antibody at 1:400 dilution.
Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody at 1:400 dilution.
Immunofluorescent analysis of Hela cells, using GAPDH Antibody.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, IP, FC
Primary Accession	P04406
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-GAPDH Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Additional Information
Gene ID	2597
Other Names	Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, 1.2.1.12, Peptidyl-cysteine S-nitrosylase GAPDH, 2.6.99.-, GAPDH {ECO:0000303\|PubMed:2987855, ECO:0000312\|HGNC:HGNC:4141}
Calculated MW	36053 Da
Application Details	WB 1:5000-1:50000 IHC 1:100-1:500 ICC/IF 1:100-1:250 IP 1:50 FC 1:50
Subcellular Localization	Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions..
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: BH-7
Immunogen	A synthesized peptide derived from human GAPDH
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GAPDH {ECO:0000303\|PubMed:2987855, ECO:0000312\|HGNC:HGNC:4141}
Function	Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis (PubMed:11724794, PubMed:3170585). Also shows nitrosylase activity, thereby playing a role in nuclear functions (PubMed:11724794, PubMed:3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1- dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
Cellular Location	Cytoplasm, cytosol. Nucleus {ECO:0000250\|UniProtKB:P04797}. Cytoplasm, perinuclear region. Membrane Cytoplasm, cytoskeleton {ECO:0000250\|UniProtKB:P04797} Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261) {ECO:0000250\|UniProtKB:P04797, ECO:0000269\|PubMed:12829261}