Anti-PTBP2 Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Western blot analysis of PTBP2 in (1) Neuro-2a cell lysate; (2) HeLa cell lysate.
Immunohistochemical analysis of paraffin-embedded human colon, using PTBP2 Antibody.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Immunofluorescent analysis using the Antibody at 1:500 dilution.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC
Primary Accession	Q9UKA9
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-PTBP2 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Additional Information
Gene ID	58155
Other Names	Polypyrimidine tract-binding protein 2, Neural polypyrimidine tract-binding protein, Neurally-enriched homolog of PTB, PTB-like protein, PTBP2 (HGNC:17662), NPTB, PTB, PTBLP
Calculated MW	57491 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200
Subcellular Localization	Nucleus.
Tissue Specificity	Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform 6 are truncated forms expressed in non- neuronal tissues..
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: ABF-16
Immunogen	A synthesized peptide derived from human PTBP2
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	PTBP2 (HGNC:17662)
Synonyms	NPTB, PTB, PTBLP
Function	RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. In addition to its function in pre-mRNA splicing, plays also a role in the regulation of translation.
Cellular Location	Nucleus {ECO:0000250\|UniProtKB:Q91Z31}.
Tissue Location	Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform 6 are truncated forms expressed in non-neuronal tissues

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.