Anti-ZNRF2 Rabbit Monoclonal Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q8NHG8
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-ZNRF2 Rabbit Monoclonal Antibody . Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Additional Information
Gene ID	223082
Other Names	E3 ubiquitin-protein ligase ZNRF2, 2.3.2.27, Protein Ells2, RING finger protein 202, RING-type E3 ubiquitin transferase ZNRF2, Zinc/RING finger protein 2, ZNRF2, RNF202
Calculated MW	24115 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:100
Subcellular Localization	Endosome membrane ; Peripheral membrane protein. Lysosome membrane ; Peripheral membrane protein. Cell junction, synapse, presynaptic cell membrane ; Peripheral membrane protein. Present in presynaptic plasma membranes in neurons.
Tissue Specificity	Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney..
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: IGB-26
Immunogen	A synthesized peptide derived from human ZNRF2
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ZNRF2
Synonyms	RNF202
Function	E3 ubiquitin-protein ligase that plays a role in the establishment and maintenance of neuronal transmission and plasticity. Ubiquitinates the Na(+)/K(+) ATPase alpha-1 subunit/ATP1A1 and thereby influences its endocytosis and/or degradation (PubMed:22797923). Acts also as a positive regulator of mTORC1 activation by amino acids, which functions upstream of the V-ATPase and of Rag-GTPases (PubMed:27244671). In turn, phosphorylation by mTOR leads to its inhibition via targeting to the cytosol allowing a self-regulating feedback mechanism (PubMed:27244671).
Cellular Location	Endosome membrane; Peripheral membrane protein. Lysosome membrane; Peripheral membrane protein. Presynaptic cell membrane; Peripheral membrane protein. Cytoplasm
Tissue Location	Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney.