癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-BubR1 BUB1B Rabbit Monoclonal Antibody
- 产品详情
- 实验流程
Application 
| WB, IHC, IP, FC |
|---|---|
| Primary Accession | O60566 |
| Host | Rabbit |
| Isotype | Rabbit IgG |
| Reactivity | Rat, Human, Mouse |
| Clonality | Monoclonal |
| Format | Liquid |
| Description | Anti-BubR1 BUB1B Rabbit Monoclonal Antibody . Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. |
| Gene ID | 701 |
|---|---|
| Other Names | Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, 2.7.11.1, MAD3/BUB1-related protein kinase, hBUBR1, Mitotic checkpoint kinase MAD3L, Protein SSK1, BUB1B, BUBR1, MAD3L, SSK1 |
| Calculated MW | 119545 Da |
| Application Details | WB 1:1000-1:5000 IHC 1:50-1:200 IP 1:30 FC 1:50 |
| Subcellular Localization | Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5. |
| Tissue Specificity | Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.. |
| Source | Eukaryota |
| Contents | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
| Clone Names | Clone: ABFG-2 |
| Immunogen | A synthesized peptide derived from human BubR1 |
| Purification | Affinity-chromatography |
| Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | BUB1B |
|---|---|
| Synonyms | BUBR1, MAD3L, SSK1 |
| Function | Component of the mitotic checkpoint complex (MCC), which inhibits APC/C and delays chromosome segregation until all chromosomes are properly attached to the mitotic spindle (PubMed:27509861). The MCC, which consists of CDC20, MAD2L1/MAD2, BUB1B/BUBR1 and BUB3, is a key player of the spindle assembly checkpoint (PubMed:11702782, PubMed:14706340, PubMed:15020684, PubMed:27509861). The MCC-bound APC/C complex is inactive and this delays the metaphase/anaphase transition (PubMed:10477750, PubMed:27509861). When the spindle assembly checkpoint is silenced, the MCC-APC/C complex recruits the E2 enzyme UBCH10 and triggers the auto-ubiquitination of CDC20 in the MCC, thereby reactivating APC/C for transition into anaphase (PubMed:27509861). MCC also monitors kinetochore activities that depend on the kinetochore motor CENPE (PubMed:15020684). Required for kinetochore localization of CENPE (PubMed:15020684). Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification (PubMed:19503101). May play a role for tumor suppression (By similarity). |
| Cellular Location | Cytoplasm. Nucleus. Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q9Z1S0}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase Kinetochore localization requires BUB1, PLK1 and KNL1 |
| Tissue Location | Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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