Anti-S100A4 Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:1000 dilution.
Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:500 dilution.
Western blot analysis of S100A4 expression in A375 cell lysate.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using S100A4 Antibody.
Immunofluorescent analysis of HeLa cells, using S100A4 Antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, IP, FC
Primary Accession	P26447
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-S100A4 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Additional Information
Gene ID	6275
Other Names	Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4, S100A4, CAPL, MTS1
Calculated MW	11729 Da
Application Details	WB 1:500-1:1000 IHC 1:100-1:500 ICC/IF 1:50-1:200 IP 1:40 FC 1:100
Tissue Specificity	Ubiquitously expressed.
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: GFG-19
Immunogen	A synthesized peptide derived from human S100A4
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	S100A4
Synonyms	CAPL, MTS1
Function	Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed:16707441, PubMed:23752197, PubMed:30713770). Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed:16707441). Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity). Also modulates the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed:23752197). Within the extracellular space, stimulates cytokine production including granulocyte colony- stimulating factor and CCL24 from T-lymphocytes (By similarity). In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed:26654597, PubMed:30713770).
Cellular Location	Secreted. Nucleus Cytoplasm {ECO:0000250\|UniProtKB:P07091}
Tissue Location	Ubiquitously expressed.