Anti-CYP2E1 Rabbit Monoclonal Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	P05181
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-CYP2E1 Rabbit Monoclonal Antibody . Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Additional Information
Gene ID	1571
Other Names	Cytochrome P450 2E1, 1.14.14.1, 4-nitrophenol 2-hydroxylase, 1.14.13.n7, CYPIIE1, Cytochrome P450-J, CYP2E1 {ECO:0000303\|PubMed:10553002, ECO:0000312\|HGNC:HGNC:2631}
Calculated MW	56849 Da
Application Details	WB 1:500-1:2000
Subcellular Localization	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: AADC-3
Immunogen	A synthesized peptide derived from human CYP2E1
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CYP2E1 {ECO:0000303\|PubMed:10553002, ECO:0000312\|HGNC:HGNC:2631}
Function	A cytochrome P450 monooxygenase involved in the metabolism of fatty acids (PubMed:10553002, PubMed:18577768). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:10553002, PubMed:18577768). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids (PubMed:10553002, PubMed:18577768). May be involved in the oxidative metabolism of xenobiotics (Probable).
Cellular Location	Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:P05182}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P05182}. Microsome membrane {ECO:0000250\|UniProtKB:P05182}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P05182}. Mitochondrion inner membrane {ECO:0000250\|UniProtKB:P05182}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P05182}. Note=Post-translationally targeted to mitochondria. TOMM70 is required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein {ECO:0000250\|UniProtKB:P05182}