Anti-PRAS40 AKT1S1 Rabbit Monoclonal Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IP, FC
Primary Accession	Q96B36
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-PRAS40 AKT1S1 Rabbit Monoclonal Antibody . Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Additional Information
Gene ID	84335
Other Names	Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate, AKT1S1 {ECO:0000312\|EMBL:AAH16043.1}
Calculated MW	27383 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 IP 1:50 FC 1:50
Subcellular Localization	Cytoplasm, cytosol. Found in the cytosolic fraction of the brain..
Tissue Specificity	Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293)..
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: ABIO-1
Immunogen	A synthesized peptide derived from human PRAS40
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	AKT1S1 {ECO:0000312\|EMBL:AAH16043.1}
Function	Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed:17277771, PubMed:17386266, PubMed:17510057, PubMed:29236692). In absence of insulin and nutrients, AKT1S1 associates with the mTORC1 complex and directly inhibits mTORC1 activity by blocking the MTOR substrate- recruitment site (PubMed:29236692). In response to insulin and nutrients, AKT1S1 dissociates from mTORC1 (PubMed:17386266, PubMed:18372248). Its activity is dependent on its phosphorylation state and binding to 14-3-3 (PubMed:16174443, PubMed:18372248). May also play a role in nerve growth factor-mediated neuroprotection (By similarity).
Cellular Location	Cytoplasm, cytosol {ECO:0000250\|UniProtKB:Q9D1F4}. Note=Found in the cytosolic fraction of the brain. {ECO:0000250\|UniProtKB:Q9D1F4}
Tissue Location	Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293)