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Anti-Vimentin Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:1000 dilution.
Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody at 1:1000 dilution.
Immunohistochemical analysis of paraffin-embedded human colon, using Vimentin Antibody.
Immunofluorescent analysis of Hela cells, using Vimentin Antibody.
Figure 1. Western blot analysis of Vimentin using anti-Vimentin antibody (M00235-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vimentin antigen affinity purified monoclonal antibody (Catalog # M00235-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vimentin at approximately 54 kDa. The expected band size for Vimentin is at 54 kDa.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, FC
Primary Accession	P08670
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-Vimentin Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Additional Information
Gene ID	7431
Other Names	Vimentin, VIM
Calculated MW	53652 Da
Application Details	WB 1:1000-1:5000 IHC 1:200-1:1000 ICC/IF 1:50-1:200 FC 1:50
Subcellular Localization	Cytoplasm.
Tissue Specificity	Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines..
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: AGF-22
Immunogen	A synthesized peptide derived from human Vimentin
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	VIM (HGNC:12692)
Function	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP- mediated recruitment to outgrowth tips (By similarity).
Cellular Location	Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250\|UniProtKB:P31000}. Cell membrane {ECO:0000250\|UniProtKB:P20152}. Cell projection, axon {ECO:0000250\|UniProtKB:P20152}
Tissue Location	Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol