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Anti-Lysozyme LYZ Rabbit Monoclonal Antibody

Figure 1. Western blot analysis of Lysozyme using anti-Lysozyme antibody (M01811).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human U937 whole cell lysates,
Lane 3: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lysozyme antigen affinity purified monoclonal antibody (Catalog # M01811) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lysozyme at approximately 15 kDa. The expected band size for Lysozyme is at 17 kDa.
Immunohistochemical analysis of paraffin-embedded mouse kidney, using Lysozyme Antibody.
Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody at 1:2000 dilution.
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:4000 dilution.
Immunohistochemical analysis of paraffin-embedded Human placenta, using the Antibody at 1:4000 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:2000 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse heart, using the Antibody at 1:2000 dilution.
Immunofluorescent analysis using the Antibody at 1:50 dilution.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, IP
Primary Accession	P61626
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-Lysozyme LYZ Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Additional Information
Gene ID	4069
Other Names	Lysozyme C, 3.2.1.17, 1, 4-beta-N-acetylmuramidase C, LYZ, LZM
Calculated MW	16537 Da
Application Details	WB 1:5000-1:10000 IHC 1:50-1:200 ICC/IF 1:50-1:200 IP 1:20
Subcellular Localization	Secreted.
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: EBO-12
Immunogen	A synthesized peptide derived from human Lysozyme
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	LYZ
Synonyms	LZM
Function	Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Cellular Location	Secreted.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol