Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:6k dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Human colon, using the Antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse lung, using the Antibody at 1:200 dilution.
Western blot analysis of Cyclin B1 expression in HeLa cell lysate.
All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human colon cancer, using Cyclin B1 Antibody.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis of Hela cells, using Cyclin B1 Antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, FC
Primary Accession	P14635
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Additional Information
Gene ID	891
Other Names	G2/mitotic-specific cyclin-B1, CCNB1, CCNB
Calculated MW	48337 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 FC 1:100
Subcellular Localization	Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: EOG-3
Immunogen	A synthesized peptide derived from human Cyclin B1
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CCNB1
Synonyms	CCNB
Function	Essential for the control of the cell cycle at the G2/M (mitosis) transition (PubMed:27030811, PubMed:17495531, PubMed:17495533). Binds and activates cyclin-dependent protein kinase CDK1/CDC2 (PubMed:41100585).
Cellular Location	Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome