Anti-Cytokeratin 8 KRT8 Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:6W dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Rat skin, using the Antibody at 1:300 dilution.
Immunohistochemical analysis of paraffin-embedded Human liver, using the Antibody at 1:600 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse heart, using the Antibody at 1:300 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Immunofluorescent analysis using the Antibody at 1:500 dilution.
Western blot analysis of Cytokeratin 8 expression in HaCaT cell lysate.
Immunohistochemical analysis of paraffin-embedded human liver, using Cytokeratin 8 Antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, IP, FC
Primary Accession	P05787
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-Cytokeratin 8 KRT8 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Additional Information
Gene ID	3856
Other Names	Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8
Calculated MW	53704 Da
Application Details	WB 1:10000-1:20000 IHC 1:50-1:200 ICC/IF 1:50-1:200 IP 1:50 FC 1:50
Subcellular Localization	Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
Tissue Specificity	Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity..
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: CIG-11
Immunogen	A synthesized peptide derived from human Cytokeratin 8
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	KRT8 (HGNC:6446)
Synonyms	CYK8
Function	Structural component of intermediate filaments in simple epithelial cells. Together with its obligate type I partner KRT18, contributes to the formation of the keratin intermediate filament network that provides mechanical stability and resilience to epithelial cells (PubMed:26823019). Also involved in maintaining epithelial integrity and protecting cells from non-mechanical stress, such as apoptosis (By similarity). Also required for ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (PubMed:26823019). In striated muscle, together with KRT19, may contribute to linking the contractile apparatus to dystrophin at the costameres (PubMed:16000376).
Cellular Location	Cytoplasm. Nucleus, nucleoplasm {ECO:0000250\|UniProtKB:Q10758}. Nucleus matrix {ECO:0000250\|UniProtKB:Q10758}. Cytoplasm, cytoskeleton
Tissue Location	Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.