Anti-P Glycoprotein ABCB1 Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Rat spleen, using the Antibody at 1:1000 dilution.
Immunohistochemical analysis of paraffin-embedded Rat heart, using the Antibody at 1:1000 dilution.
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:2000 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:1000 dilution.
Western blot analysis of P Glycoprotein expression in HeLa cell lysate.
Immunohistochemical analysis of paraffin-embedded human liver, using P Glycoprotein Antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P08183
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-P Glycoprotein ABCB1 Rabbit Monoclonal Antibody . Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Additional Information
Gene ID	5243
Other Names	ATP-dependent translocase ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, 7.6.2.2, P-glycoprotein 1, Phospholipid transporter ABCB1, 7.6.2.1, CD243, ABCB1 (HGNC:40), MDR1, PGY1
Calculated MW	141479 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200
Subcellular Localization	Cell membrane ; Multi-pass membrane protein.
Tissue Specificity	Expressed in liver, kidney, small intestine and brain.
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: FDB-1
Immunogen	A synthesized peptide derived from human P Glycoprotein
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ABCB1 (HGNC:40)
Synonyms	MDR1, PGY1
Function	Translocates drugs and phospholipids across the membrane (PubMed:2897240, PubMed:35970996, PubMed:8898203, PubMed:9038218, PubMed:35507548). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:35970996, PubMed:9038218).
Cellular Location	Cell membrane; Multi-pass membrane protein {ECO:0000255\|PROSITE-ProRule:PRU00441} Apical cell membrane. Cytoplasm Note=ABCB1 localization is influenced by C1orf115 expression levels (plasma membrane versus cytoplasm). Localized to the apical membrane of enterocytes (PubMed:28408210).
Tissue Location	Expressed in small intestine (PubMed:28408210). Expressed in liver, kidney and brain.