Anti-Growth Hormone GH1 Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human placenta, using Growth Hormone Antibody.
Figure 1. Western blot analysis of GH1 using anti-GH1 antibody (M00851-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GH1 antigen affinity purified monoclonal antibody (Catalog # M00851-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GH1 at approximately 22 kDa. The expected band size for GH1 is at 25 kDa.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IP
Primary Accession	P01241
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Human
Clonality	Monoclonal
Format	Liquid
Description	Anti-Growth Hormone GH1 Rabbit Monoclonal Antibody . Tested in WB, IHC, IP applications. This antibody reacts with Human.

Additional Information
Gene ID	2688
Other Names	Somatotropin, Growth hormone, GH, GH-N, Growth hormone 1, Pituitary growth hormone, GH1
Calculated MW	24847 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 IP 1:20
Subcellular Localization	Secreted.
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: AADG-7
Immunogen	A synthesized peptide derived from human Growth Hormone
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GH1
Function	Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Cellular Location	Secreted

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.