癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody
- 产品详情
- 实验流程
Application 
| WB, IHC, IF, ICC, FC |
|---|---|
| Primary Accession | Q99ZW2 |
| Host | Rabbit |
| Isotype | Rabbit IgG |
| Clonality | Monoclonal |
| Format | Liquid |
| Description | Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Recombinant fragment, Streptococcus pyogenes. |
| Other Names | CRISPR-associated endonuclease Cas9/Csn1 {ECO:0000255|HAMAP-Rule:MF_01480}, 3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01480}, SpCas9, SpyCas9, cas9 {ECO:0000255|HAMAP-Rule:MF_01480, ECO:0000303|PubMed:22745249} |
|---|---|
| Calculated MW | 158441 Da |
| Application Details | WB 1:1000-1:5000 IHC 1:50-1:200 ICC/IF 1:50-1:200 FC 1:50 |
| Source | Bacteria |
| Contents | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
| Clone Names | Clone: ABCI-3 |
| Immunogen | Recombinant fragment derived from Streptococcus pyogenes. |
| Purification | Affinity-chromatography |
| Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | cas9 {ECO:0000255|HAMAP-Rule:MF_01480, ECO:0000303|PubMed:22745249} |
|---|---|
| Function | CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids) (PubMed:21455174). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing (PubMed:24270795). Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed 3'-5' exonucleolytically. DNA-binding requires protein and both gRNAs, as does nuclease activity. Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. DNA strand separation and heteroduplex formation starts at PAM sites; PAM recognition is required for catalytic activity (PubMed:24476820). Confers immunity against a plasmid with homology to the appropriate CRISPR spacer sequences (CRISPR interference) (PubMed:21455174). |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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