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Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody

Figure 1. Western blot analysis of MPO using anti-MPO antibody (M00372).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPO antigen affinity purified monoclonal antibody (Catalog # M00372) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPO at approximately 84 kDa. The expected band size for MPO is at 84 kDa.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC
Primary Accession	P05164
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Human
Clonality	Monoclonal
Format	Liquid
Description	Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Additional Information
Gene ID	4353
Other Names	Myeloperoxidase, MPO, 1.11.2.2, Myeloperoxidase, 89 kDa myeloperoxidase, 84 kDa myeloperoxidase, Myeloperoxidase light chain, Myeloperoxidase heavy chain, MPO (HGNC:7218)
Calculated MW	83869 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200
Subcellular Localization	Lysosome.
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: AOCF-13
Immunogen	A synthesized peptide derived from human Myeloperoxidase
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MPO {ECO:0000303\|PubMed:21880720, ECO:0000312\|HGNC:HGNC:7218}
Function	Peroxidase that plays a central role in the host defense system of polymorphonuclear leukocytes by mediating both (1) formation of neutrophil extracellular trap (NETs) and (2) microbicidal activity (PubMed:16125131, PubMed:17438335, PubMed:20974672, PubMed:20974816, PubMed:22131345, PubMed:25066128, PubMed:28574339, PubMed:40963017, PubMed:4978299, PubMed:6295491). Promotes NET formation by mediating chromatin disassembly: translocates to the nucleus and specifically binds to nucleosomes, both as monomer and homodimer, leading to nucleosome unstacking and initial chromatin decondensation (PubMed:40963017). Homodimers clash with one end of the nucleosomal DNA, leading to DNA unwrapping, initiating complete disassembly of nucleosomes and chromatin transformation into NETs in an ATP- independent manner (PubMed:40963017). NETs, which are mainly composed of DNA fibers and globular proteins, are then extruded into the extracellular space by neutrophils to trap pathogens and release antimicrobial proteins to destroy them (PubMed:40963017). Participates to the microbicidal activity against a wide range of organisms by acting as a peroxidase that catalyzes the formation of oxidants in presence of hydrogen peroxide (PubMed:16125131, PubMed:17074761, PubMed:17438335, PubMed:21880720, PubMed:22131345, PubMed:22352991, PubMed:22902565, PubMed:24194519, PubMed:33273015, PubMed:9359420, PubMed:9922160). Mediates the formation of hypohalous acids, mainly hypochlorous acid (HOCl) in physiologic situations, that greatly enhance polymorphonuclear leukocyte microbicidal activity (PubMed:16125131, PubMed:17074761, PubMed:17438335, PubMed:19608745, PubMed:21880720, PubMed:22902565, PubMed:24194519, PubMed:9359420, PubMed:9922160). In addition to hypochlorous acid, catalyzes formation of hypobromous acid (HOBr), hypoiodous acid (HOI) and hypothiocyanous acid (HOSCN) (PubMed:1318692, PubMed:16125131, PubMed:17438335, PubMed:19608745, PubMed:6295491, PubMed:9359420). Also catalyzes oxidation of nitrite into the highly reactive nitrogen dioxide radical (PubMed:10777476, PubMed:8385644, PubMed:9450756). Formation of oxidants are widely believed to be responsible for much of the anti- bactericidal activity of neutrophils (PubMed:8385644, PubMed:9450756). Oxidants, such as hypochlorous acid or nitrogen dioxide radical, can also oxidize amino acid residues on proteins and generate chlorination and nitration post-translational modifications, respectively (PubMed:15314690, PubMed:15326314, PubMed:15498770, PubMed:15574409, PubMed:16091367, PubMed:7622459). Chlorination and nitration of the lipid-free form of APOA1 impairs cholesterol transport (PubMed:15326314, PubMed:15498770, PubMed:15574409). Superoxides generated by MPO can also promote dioxygenation of tryptophan residues on proteins (PubMed:16091367, PubMed:40081572). Also able to oxidize melatonin into N1-acetyl-N2-formyl-5-methoxykynuramine either in presence of hydrogen peroxide or superoxide (PubMed:15636586, PubMed:16148002). Oxidizes urate into 5-hydroxyisourate (PubMed:21266577). Functions as a nitric oxide (NO) oxidase during inflammation, by catalytically consuming NO, impairing NO's ability to maintain vascular tone and function (PubMed:12089442). May also mediate the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1- microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed:25698971).
Cellular Location	Lysosome. Cytolytic granule. Cytoplasmic vesicle, phagosome. Secreted. Nucleus. Chromosome Note=Localizes to azurophil granules, a type of primary lysosome found in neutrophils and monocytes that contains antimicrobial proteins (PubMed:25066128, PubMed:2981589, PubMed:6095812, PubMed:6192815). A substantial fraction is also constitutively secreted by normal bone marrow granulocyte precursors (PubMed:6095812). During formation of neutrophil extracellular traps (NETs), translocates into the nucleus and associates to chromatin, leading to chromatin dislocation and its transformation into NETs (PubMed:25066128, PubMed:40963017). Core component of NETs (PubMed:22131345, PubMed:32434865, PubMed:40963017)
Tissue Location	Specifically expressed in neutrophils and monocytes.