Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody

Western blot analysis of myosin light chain kinase expression in HUVEC cell lysate.
Immunohistochemical analysis of paraffin-embedded human colon, using Myosin light chain kinase Antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, FC
Primary Accession	Q15746
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Additional Information
Other Names	Myosin light chain kinase, smooth muscle, MLCK, smMLCK, 2.7.11.18, Kinase-related protein, KRP, Telokin, Myosin light chain kinase, smooth muscle, deglutamylated form, MYLK (HGNC:7590), MLCK, MLCK1, MYLK1
Calculated MW	210715 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 FC 1:50
Subcellular Localization	Cytoplasm. Cell projection, lamellipodium. Cleavage furrow. Cytoplasm, cytoskeleton. Localized to stress fibers during interphase and to the cleavage furrow during mitosis.
Tissue Specificity	Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues. Accumulates in well differentiated enterocytes of the intestinal epithelium in response to tumor necrosis factor (TNF)..
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: G-13
Immunogen	A synthesized peptide derived from human Myosin light chain kinase
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.