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Anti-Cardiac FABP Fabp3 Antibody Picoband™ (monoclonal, 6C4)

Figure 1. Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody (M01734).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates,
Lane 2: mouse heart tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cardiac FABP antigen affinity purified monoclonal antibody (Catalog # M01734) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Figure 2. IHC analysis of Cardiac FABP using anti-Cardiac FABP antibody (M01734).
Cardiac FABP was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Cardiac FABP Antibody (M01734) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. Sandwich ELISA - Recombinant rat Cardiac FABP protein standard curve.
Use in combination with reagents from Rat Cardiac FABP ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1623).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	P07483
Host	Mouse
Isotype	Mouse IgG1
Reactivity	Rat, Mouse
Clonality	Monoclonal
Format	Lyophilized
Description	Anti-Cardiac FABP Fabp3 Antibody Picoband™ (monoclonal, 6C4) . Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Additional Information
Gene ID	79131
Other Names	Fatty acid-binding protein, heart, Fatty acid-binding protein 3, Heart-type fatty acid-binding protein, H-FABP, Fabp3
Calculated MW	14775 Da
Application Details	Western blot, 0.1-0.5 µg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1 µg/ml ELISA (Cap), 1-5 µg/ml
Subcellular Localization	Cytoplasm.
Tissue Specificity	Heart, but also skeletal muscle, kidney, brain and mammary gland.
Source	Eukaryota
Contents	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clone Names	Clone: 6C4
Immunogen	E. coli-derived rat Cardiac FABP recombinant protein (Position: A2-A133).
Cross Reactivity	No cross-reactivity with other proteins.
Storage	Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	Fabp3
Function	FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
Cellular Location	Cytoplasm.
Tissue Location	Heart, but also skeletal muscle, kidney, brain and mammary gland

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Heart-type fatty acid binding protein (hFABP), also known as mammary-derived growth inhibitor, is a protein that in humans is encoded by the FABP3 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer. Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP.

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¥ 3,367.00

Cat# ABO14334

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100 µg

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Anti-Cardiac FABP Fabp3 Antibody Picoband™ (monoclonal, 6C4)

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