购物车 0
注册 | 登录
0512-88856768
联系我们
点击这里给我发消息
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   信号转导   >   Anti-RRM2 Monoclonal Antibody   

Anti-RRM2 Monoclonal Antibody

     
  • 1 - Anti-RRM2 Monoclonal Antibody ABO14602
    Figure 1. Western blot analysis of RRM2 using anti-RRM2 antibody (M01978).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human SIHA whole cell lysates,
    Lane 3: human U251 whole cell lysates,
    Lane 4: human MCF-7 whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRM2 antigen affinity purified monoclonal antibody (Catalog # M01978) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRM2 at approximately 45 kDa. The expected band size for RRM2 is at 45 kDa.
  • 产品详情
  • 实验流程
Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, ICC, IP
Primary Accession P31350
Host Rabbit
Isotype Rabbit IgG
Reactivity Human
Clonality Monoclonal
Format Liquid
Description Anti-RRM2 Monoclonal Antibody . Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.
Additional Information
Gene ID 6241
Other Names Ribonucleoside-diphosphate reductase subunit M2, 1.17.4.1, Ribonucleotide reductase small chain, Ribonucleotide reductase small subunit, RRM2, RR2
Calculated MW 44878 Da
Application Details WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
Source Eukaryota
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: AEAE-18
Immunogen A synthesized peptide derived from human RRM2 Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name RRM2
Synonyms RR2
Function Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
Cellular Location Cytoplasm. Nucleus. Note=Localized to the cytoplasm in S phase cells. May localize to the nucleus in G2 phase cells
Research Areas
Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol
FeedBack

终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问，联系：0512-88856768 tech-china@abcepta.com.


我要评价

¥ 3,367.00
Cat# ABO14602
Size:
100 µl
Price:
¥ 3,367.00
Quantity:
reduce add
Availability:
询价

相关产品

ABO14602: Anti-RRM2 Monoclonal Antibody
ABO12503: Anti-RRM2 Picoband Antibody
AP91639: RRM2 Antibody
查看更多产品
抗体制备服务
产品
新品速递
Crown抗体
一抗
产品文献引用
订单
联系我们
关于我们
新闻&活动
人才招聘
支持
技术支持
工具
相关链接
文献资料
促销活动
在线留言
客户售后反馈
Copyright © 2026 Abcepta Biotech Ltd. Co. 苏ICP备20009897号
地址：中国（江苏）自由贸易试验区苏州片区苏州工业园区双圩路9号1幢
电话：0512-88856768