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>   首页   >   产品   >   一抗   >   信号转导   >   Anti-Zyxin Monoclonal Antibody   

Anti-Zyxin Monoclonal Antibody

     
  • 1 - Anti-Zyxin Monoclonal Antibody ABO14693
    Figure 1. Western blot analysis of Zyxin using anti-Zyxin antibody (M02365).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human MCF-7 whole cell lysates,
    Lane 2: human PC-3 whole cell lysates,
    Lane 3: human SIHA whole cell lysates,
    Lane 4: rat lung tissue lysates,
    Lane 5: mouse lung tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Zyxin antigen affinity purified monoclonal antibody (Catalog # M02365) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Zyxin at approximately 78 kDa. The expected band size for Zyxin is at 61 kDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, IF, ICC, IP, FC
Primary Accession Q15942
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Zyxin Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 7791
Other Names Zyxin, Zyxin-2, ZYX
Calculated MW 61277 Da
Application Details WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:500
Source Eukaryota
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: AFOG-26
Immunogen A synthesized peptide derived from human Zyxin.
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name ZYX
Function Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion- stimulated changes in gene expression (By similarity).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Cell junction, focal adhesion. Note=Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1
Research Areas
Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol
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¥ 3,367.00
Cat# ABO14693
Size:
100 µl
Price:
¥ 3,367.00
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相关产品

ABO14693: Anti-Zyxin Monoclonal Antibody
AP95239: Recombinant Anti-Zyxin Rabbit mAb
AT4653a: ZYX Antibody (monoclonal) (M01)
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