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Anti-His Tag Antibody Picoband™ (monoclonal, 19b10)

     
  • 1 - Anti-His Tag Antibody Picoband™ (monoclonal, 19b10) ABO14865
    Figure 1. Western blot analysis of His Tag using anti-His Tag antibody (M30975-1).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
    Lane 1: recombinant mouse His Tag protein 10ng,
    Lane 1: recombinant mouse His Tag protein 5ng,
    Lane 1: recombinant mouse His Tag protein 2.5ng,
    Lane 1: recombinant mouse His Tag protein 1.25ng.
    After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-His Tag antigen affinity purified monoclonal antibody (Catalog # M30975-1) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
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Product Information
Host Mouse
Isotype Mouse IgG2b
Clonality Monoclonal
Format Lyophilized
Description Anti-His Tag Antibody Picoband™ (monoclonal, 19b10) . Tested in WB application. This antibody reacts with all species.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500 µg/ml.
Additional Information
Application Details Western blot, 0.1-0.5 µg/ml
Contents Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clone Names Clone: 19b10
Immunogen A synthetic peptide corresponding to a sequence of His Tag.
Cross Reactivity No cross-reactivity with other proteins.
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas
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