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Anti-beta Tubulin Mouse Monoclonal Antibody

Western blot analysis of beta Actin expression in (1) A431 cell lysate; (2) Rat brain lysate; (3) Mouse kidney lysate; (4) Chicken lung lysate; (5) Hamster spleen lysate with beta Tubulin Mouse Monoclonal Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using beta Tubulin Mouse Monoclonal Antibody.
Immunofluorescent analysis of Hela cells, using beta Tubulin mAb.
Figure 1. Western blot analysis of beta Tubulin using anti-beta Tubulin antibody (M05613-5).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-beta Tubulin antigen affinity purified monoclonal antibody (Catalog # M05613-5) at 1:3000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta Tubulin at approximately 50 kDa. The expected band size for beta Tubulin is at 50 kDa.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC
Primary Accession	P07437
Host	Mouse
Isotype	IgG1
Reactivity	Rat, Human, Mouse, Hamster, Chicken
Clonality	Monoclonal
Format	Liquid
Description	Anti-beta Tubulin Mouse Monoclonal Antibody . Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat, Chicken, Hamster.

Additional Information
Gene ID	203068
Other Names	Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5
Calculated MW	49671 Da
Application Details	WB 1:3000-1:10000 IHC 1:50-1:200 ICC/IF 1:50-1:200
Source	Eukaryota
Contents	Mouse IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: 17T13
Immunogen	A synthesized peptide derived from human beta Tubulin
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TUBB
Synonyms	TUBB5
Function	Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Cellular Location	Cytoplasm, cytoskeleton
Tissue Location	Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol