Anti-Phospho-ERK1 (T202) + ERK2 (T185) Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Western blot analysis of Phospho-ERK1 (T202) + ERK2 (T185) expression in (1) NIH/3T3 cell lysate; (2) NIH/3T3 cell lysate treated with PDGF.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, IP
Primary Accession	P27361/P28482
Host	Rabbit
Isotype	IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-Phospho-ERK1 (T202) + ERK2 (T185) Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Additional Information
Application Details	WB 1:500-1:1000 IHC 1:50-1:200 ICC/IF 1:50-1:200 IP 1:40
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: 18M06
Immunogen	A synthesized peptide derived from human Phospho-ERK1 (T202) + ERK2 (T185)
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.